In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver.

O’Connor S, Cohen P, et al.

Laboratory of Molecular Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.

Methods in molecular biology (Clifton, N.J.). 2017.

Inter-organ communication is central to mammalian metabolism, allowing for the coordination of crucial homeostatic processes such as food intake, body weight, and blood glucose. Dissecting this process at a cellular and molecular level requires the establishment of a reductionist system in which cell types of interest can be investigated as the source and target of biologically relevant signals. Here, we describe a system to study the interaction between adipocytes and hepatocytes using either conditioned media from cultured adipocytes or co-culture of hepatocytes with adipose explants. These methods have the potential to identify novel polypeptides and metabolites involved in the adipose-liver metabolic axis.


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