A Preliminary Study of the Action of Virtosomes From Non-Dividing Cells on Tumour Cell Replication in Vitro and in Vivo.

Garcia-Arranz M, Garcia-Olmo D, Vega-Clemente L et al.

Fundación Instituto de Investigación Sanitaria Fundación Jiménez Díaz – Nuevas Terapias Madrid, Madrid,Spain Universidad Autónoma de Madrid – Surgery Madrid, Madrid, Spain.

Anti-cancer agents in medicinal chemistry. Feb 2017.

A DNA-RNA-lipoprotein complex, termed a virtosome, is released spontaneously from healthy human, other mammalian, avian, amphibian and plant cells in a regulated and energy-dependent manner. Studies on human and mouse lymphocytes, hepatocytes, NIH 3T3 cells and mouse tumour cell lines have shown that virtosomes may be acting as inter-cellular messengers. In particular, virtosomes from non-dividing cells blocked 3H-thymidine incorporation into DNA in tumour cell lines.Study of the effect of virtosomes on tumors "in vivo" and "in vitro" Methods and Results. The present study shows in vitro effects of virtosomes isolated from rat liver, essentially non-dividing cell populations, on cultures of healthy smooth muscle cells (SMC), human umbilical vein endothelial cells (HUVEC), human fibroblasts (h-fibroblasts) and mouse embryonic fibroblasts (NIH-3T3) together with two tumour cell lines, human Duke’s type B colorectal adenocarcinoma cells (SW480) and human connective tissue fibrosarcoma cells (HT1080). Multiplication of all cell lines was inhibited by the liver virtosomal preparation even with various dilutions of the extract (100 – 0.5%). In an in vivo study, tumours were initiated by subcutaneous injection of 1.0 x 106 DHD/K12-PROb cells in 6 weeks old male BIDX rats. Visible tumours (<1cm) appeared after 3 weeks and lung metastases after 8 weeks (80%). Virtosomes introduced via a tail vein on tumour initiation resulted in a reduction in tumour size and number.Virtosomal preparation from a non-dividing cell population inhibited cell division, reduced tumour size and partially blocked metastasis.